Manipal Life Sciences Centre (MLSC), constitute institution of Manipal University (MU) is recognized for its education, research and diagnostic activities. MLSC is identified as TIFAC-CORE (Technology Information, Forecasting and Assessment Council Centres of Relevance and Excellence) under vision 2020 in the area of Pharmacogenomics. The fundamental laboratory skills and knowledge are vital for a student of biotechnology. The present manual is class-tested and offers clear and concise laboratory fundamental experiment protocols that reinforce understanding of concepts, where emphasis is on foundation skill. The faculty members of MLSC have compiled various protocols in subjects such as Organic Chemistry, Inorganic Chemistry, Physical Chemistry, Biochemistry, Molecular Biology, Cell Biology, Toxicology and Plant Tissues Culture in this Manipal Laboratory Manual for Biotechnologists. This, we believe, will serve as a guide that can be used for quick reference.